Primary charge separation routes in the BChl:BPhe heterodimer reaction centers of Rhodobacter sphaeroides.

Trapping the P+B(L)- initial intermediate state of charge separation in photosynthetic reaction centers from Rhodobacter capsulatus.

The short-lived (<1 ps) initial intermediate state P(+)B(L)(-) in the photoinduced charge separation process of the bacterial photosynthetic reaction center has been trapped in two D(LL)-based Rhodobacter capsulatus mutants that have Tyr at position M208 and lack the bacteriopheophytin electron acceptor H(L). Transient state P(+)B(L)(-) is characterized by a 1017 nm bacteriochlorophyll anion ab...

Electron transfer in a genetically modified bacterial reaction center containing a heterodimer.

Time-resolved optical measurements encompassing the femtoseconds to seconds time scales have been used to investigate Rhodobacter capsulatus reaction centers (RCs) in which the histidine residue at position 200 on the M polypeptide has been changed to a leucine by site-directed mutagenesis. The HisM200----Leu RC, which has a heterodimer consisting of a bacteriochlorophyll and a bacteriopheophyt...

Supporting Information Early bacteriopheophytin reduction in charge separation in reaction centers of Rhodobacter sphaeroides

Stark effect spectroscopy of Rhodobacter sphaeroides and Rhodopseudomonas viridis reaction centers.

The nature of the initially excited state of the primary electron donor or special pair has been investigated by Stark effect spectroscopy for reaction centers from the photosynthetic bacteria Rhodopseudomonas viridis and Rhodobacter sphaeroides at 77 K. The data provide values for the magnitude of the difference in permanent dipole moment between the ground and excited state, [unk]Deltamu[unk]...

Electric Field Modulation of the Fluorescence from Rhodobacter Sphaeroides Reaction Centers

The fluorescence intensity from randomly oriented Rhodobacter (Rb.) sphaeroides photosynthetic reaction centers at 77 K increases in an applied electric field. This is ascribed to a net reduction in the rate of the initial electron transfer reaction which competes with fluorescence due to the effect of the field on the free energy of charge separation. Quantitative analysis of the fluorescence ...